Cloning and expression of T4 DNA polymerase

  1. T C Lin,
  2. J Rush,
  3. E K Spicer, and
  4. W H Konigsberg
  1. Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, New Haven, CT 06510.

Abstract

The structural gene coding for bacteriophage T4 DNA polymerase (gene 43) has been cloned into inducible plasmid vectors, which provide a source for obtaining large amounts of this enzyme after induction. The T4 DNA polymerase produced in this fashion was purified by an innovative three-step procedure and was fully active.

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  1. PNAS October 1, 1987 vol. 84 no. 20 7000-7004
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