Magnetic Circular Dichroism Studies XXXV. A Comparison of Cytochromes P-450 and P-448*

  1. John H. Dawson†,
  2. Peter M. Dolinger†,
  3. James R. Trudell‡,
  4. Günter Barth†,
  5. Robert E. Linder†,
  6. Edward Bunnenberg†, and
  7. Carl Djerassi†,§
  1. †Department of Chemistry, Stanford University, Stanford, California 94305
  2. ‡Department of Anesthesia, Stanford University School of Medicine, Stanford, California 94305

Abstract

Cytochromes P-450 and P-448 in microsomal suspensions have been shown to be spectrally distinct by magnetic circular dichroism spectroscopy. Furthermore, this technique can be used to measure induction of these two cytochromes by phenobarbital and 3-methyl-cholanthrene. Magnetic circular dichroism spectroscopy is thus at least as useful as difference spectroscopy for the investigation of P-450 and P-448 and more informative because the presence of cytochrome b 5 and hemoglobin can be detected concurrently. We have also shown that the molar magnetic ellipticity for reduced + CO treated cytochrome P-450 of Pseudomonas grown on camphor is of similar value to that of reduced + CO treated microsomal P-450 and P-448.

Footnotes

  • ↵ § Author to whom correspondence should be addressed.

  • ↵ * Part XXXIV is a manuscript submitted for publication by G. Barth, J. H. Dawson, P. M. Dolinger, R. E. Linder, E. Bunnenberg, and C. Djerassi.

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  1. PNAS November 1, 1974 vol. 71 no. 11 4594-4597
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