Characterization of the End Groups of RNA of Rous Sarcoma Virus

Abstract

The 3′ terminus of the 30-35S RNA of Rous sarcoma virus is adenosine. Its amount indicates an average molecular weight for that RNA of about 3 × 10⁶. The 5′ terminus of 30-35S RNA of Rous sarcoma virus was not di- or triphosphorylated, whether isolated by the standard procedure or from virus collected within 3 min of its release from the cells.

• 3′-terminal nucleoside analysis
• 5′-phosphate analysis