Membrane Structure: Lipid-Protein Interactions in Microsomal Membranes*
Abstract
The relationships of phospholipid to membrane structure and function were examined in hepatic microsomes. Findings indicate that normal microsomal membrane structure is dependent on lipid-protein interactions and that it correlates closely with glucose-6-phosphatase activity. Modification of most phospholipid with phospholipase-C is associated with widening of the membrane which can be reversed following readdition of phospholipid.
Footnotes
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↵ † Requests for reprints may be addressed to Dr. Trump, Department of Pathology, Duke University, Durham, North Carolina.
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↵ ‡ Present address: Department of Physiology, Emory University, Atlanta, Georgia.
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↵ § Present address: Department of Biochemistry, University of Tennessee Medical Units, Memphis, Tennessee.
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↵ * Supported in part by grants from NIH (AM-10698 to B. F. T., FO5-TW1242-02 to A. U. A., GM-06628 to W. L. B.), and a grant from the Finnish National Research Council for Medical Sciences to A. U. A.
