Electrophoretically Homogeneous Anti-DNP Antibodies with Restricted Isoelectric Points Elicited in Mice by Immunization with the Antigen Papain-S-DNPL*

  1. Gary N. Trump† and
  2. S. J. Singer‡
  1. DEPARTMENT OF BIOLOGY, UNIVERSITY OF CALIFORNIA, SAN DIEGO (LA JOLLA)

Abstract

The primary immunization of outbred mice with the antigen Pap-S-DNPL results in the generation of low titers of anti-DNP antibody which in about one-third of the responding animals is as homogeneous as a myeloma protein by the combined criteria of (a) isoelectric focusing in gels and (b) gel electrophoresis of the antibody light chains. The electrophoretically homogeneous antibodies show a marked restriction of isoelectric points near pH 5.0. Such marked selectivity of the antihapten antibodies appears to result from the chemical and structural homogeneity of the Pap-S-DNPL antigen.

Footnotes

  • ↵ † U.S. Public Health Service Postdoctoral Fellow, 1967-1969. Present address: Department of Genetics, University of Hawaii.

  • ↵ ‡ To whom reprint requests should be addressed.

  • ↵ * This work was supported by grants AI-06659 and GM-12027 from the U.S. Public Health Service.

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  1. PNAS June 1, 1970 vol. 66 no. 2 411-418
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