Dolichol Monophosphate Glucose: An Intermediate in Glucose Transfer in Liver*
Abstract
The microsomal fraction of liver has been found to catalyze glucose transfer from UDPG to a lipid acceptor which appears to be identical to the compound obtained by chemical phosphorylation of dolichol. The substance formed (dolichol monophosphate glucose) is acid labile and yields 1,6-anhydroglucosan by alkaline treatment. It can be used as substrate by the enzyme system yielding a glucoprotein which is subsequently hydrolyzed to glucose.
Footnotes
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↵ † Career investigator of the Consejo Nacional de Investigaciones Cientificas y Técnicas (Argentina).
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↵ ‡ Reprints may be addressed to Dr. Leloir, Instituto de Investigaciones Bioquímicas „Fundación Campomar,” 2490 Obligado, Buenos Aires (28), Argentina.
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↵ * Supported in part by grants from the U.S. Public Health Service (GM 03442) and the Consejo Nacional de Investigaciones Cientificas y Técnicas (Argentina).
