NUCLEAR MAGNETIC RESONANCE STUDIES OF THE STRUCTURE AND BINDING SITES OF ENZYMES, X. PREPARATION OF SELECTIVELY DEUTERATED ANALOGS OF STAPHYLOCOCCAL NUCLEASE
Abstract
Five different selectively deuterated analogs of staphylococcal nuclease (Foggi) were prepared having all but a selected few hydrogen atoms in the enzyme molecule replaced by deuterium. The 1H-NMR spectra of these analogs are extremely simplified, since they consist of lines arising only from the remaining protonated sites.
