Dachshund inhibits oncogene-induced breast cancer cellular migration and invasion through suppression of interleukin-8

DACH1 inhibits wound healing and persistence of migratory directionality. (A) Migration assays of MCF10A-Raf transformed cells. Cells were transduced with either retroviral vectors encoding GFP or DACH1. Data are mean ± SEM of five separate experiments for either transwell migration (A), wound closure assays (B) or phase contrast video microscopy (C). The velocity and distance migrated was determined for individual MCF10A-Raf cells transduced with either control vector or DACH1. Data mean ± SEM of n > 5 separate events. (D) Migration assays of MCF10A-Ras/ErbB2 cells transformed with either control (GFP) or DACH1. Data mean ± SEM of n > 5 separate experiments (Left) or transwell assays were conducted either in the presence of supernatant derived from cells expressing GFP, DACH1 or a mutant of the DACH1 DS domain (DACH1 ΔDS) (Right). Migration is reduced in media derived from DACH1 tranduced cells. Schematic representation of DACH1 and DACH1 ΔDS domain. *, P < 0.01.