Dachshund inhibits oncogene-induced breast cancer cellular migration and invasion through suppression of interleukin-8

DACH1 inhibits oncogene-induced migration of breast cancer cells. (A) MCF10A or MCF10A-Ras cells were transduced with retroviral expression vectors encoding GFP or DACH1-IRES-GFP and subjected to GFP-FACS sorting with subsequent analysis of cell migration by transwell assay. The data are shown as mean ± SEM of the number of cells migrated in n > 5 separate experiments. Crystal violet dye staining of MCF10A-Ras cells that migrated in the transwell assays is shown. (B) Videomicroscopy of individual cells were quantitated to determine the distance and direction of cellular migration of MCF10A-Ras cells transfected with either control (GFP) or DACH1 expression vector. (C) MCF10A-ErbB2 (NeuT) transformed cells were analyzed for migration. The mean data for the number of cells migrated per field is shown for MCF-10A oncogene-transformed cells transduced with retroviral vector encoding GFP or DACH1-IRES-GFP. (D) Western blot analysis of MCF10A-ErbB2 cells shown in C with antibodies as indicated. (E) MCF10A-c-Myc cells transduced with DACH1 or control vector analyzed for migration as shown in A. *, P < 0.01.