13C isotopologue perturbation studies of Listeria monocytogenes carbon metabolism and its modulation by the virulence regulator PrfA

Eisenreich et al. 10.1073/pnas.0507580103.

Supporting Information

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Supporting Table 1
Supporting Table 2
Supporting Figure 7
Supporting Figure 8
Supporting Figure 9
Supporting Table 3
Supporting Figure 10
Supporting Table 4
Supporting Table 5
Supporting Table 6
Supporting Table 7
Supporting Table 8
Supporting Table 9




Supporting Figure 7

Fig. 7. 13C NMR signal of C-6/C-8 of Tyr from L. monocytogenes EGD grown with [U-13C6]glucose. 13C-coupled signals and their corresponding 13C-coupling patterns and isotopologues are indicated by colors. The colored bars symbolize adjacent 13C-atoms.





Supporting Figure 8

Fig. 8. Isotopologue composition of amino acids from Listeria monocytogenes strains grown on medium containing [U-13C6]glucose. The colored bars symbolize adjacent 13C atoms. The line widths were adjusted according to the respective abundances of each isotopologue. Isolated 13C atoms (with adjacent 12C atoms) are indicated by filled circles. The numbers indicate 13C enrichments in mol %.





Supporting Figure 9

Fig. 9. Schematic overview of the different Ser production routes and fluxes. Illustration of the flux distribution predictions (Table 9, Ser Scn II) by yana. Elementary mode analysis revealed two alternative routes for Ser production, one using the Ser dehydrogenase and the other using the phosphoserine aminotransferase. The predicted flux coefficients (fraction of the total flux in the system) were supposed to be equally distributed between the two alternative routes, a hypothesis which was verified through the isotopologue data. The diagram shows a schematic overview of the network regarding Ser only and is not meant to be complete.





Supporting Figure 10

Fig. 10. 13C NMR signals of methyl groups in valine, isoleucine, and leucine isolated from L. monocytogenes EGDe (A), L. monocytogenes DprfA (B), and L. monocytogenes DprfApPRFA* (C) grown with [U-13C6]glucose.

This Article

  1. Published online before print February 6, 2006, doi: 10.1073/pnas.0507580103 PNAS February 14, 2006 vol. 103 no. 7 2040-2045
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