Histamine signaling through the H2 receptor in the Peyer’s patch is important for controlling Yersinia enterocolitica infection
- Departments of *Molecular Microbiology and
- ‡Pediatrics, Washington University School of Medicine, 660 South Euclid Avenue, St. Louis, MO 63110
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Edited by Stanley Falkow, Stanford University, Stanford, CA, and approved April 12, 2006 (received for review December 2, 2005)
Abstract
Enteric pathogens such as Yersinia enterocolitica readily colonize and induce disease within the lymphatic tissues of the small intestine. To gain a comprehensive view of the host response to pathogens within these tissues, we determined the transcriptional profiles of intestinal lymphatic tissue infected with Y. enterocolitica. Expression analysis using Affymetrix GeneChips revealed a complex host response in the Peyer’s patches and mesenteric lymph nodes after oral infection with Y. enterocolitica. Interestingly, histidine decarboxylase (Hdc) was significantly up-regulated in response to Y. enterocolitica infection. HDC is the enzyme solely responsible for the production of the biogenic amine histamine. Although histamine is well known for its role in allergy and for its effects on immunity and inflammation, little is known about its role or specific histamine receptors during the host response to bacterial infection. In this study, we provide evidence that histamine signaling through the histamine H2 but not the H1 receptor is important for controlling Y. enterocolitica infection within the Peyer’s patches and mesenteric lymph nodes of mice.
Footnotes
- §To whom correspondence should be addressed. E-mail: virginia{at}borcim.wustl.edu
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↵ †Present address: Department of Microbiology and Immunology, University of Texas Health Sciences Center, 7703 Floyd Curl Drive, MC7758, San Antonio, TX 78229-3900.
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Author contributions: S.A.H., P.H.D., and V.L.M. designed research; S.A.H. and P.H.D. performed research; S.A.H., P.H.D., and V.L.M. analyzed data; and S.A.H. and V.L.M. wrote the paper.
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Conflict of interest statement: No conflicts declared.
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This paper was submitted directly (Track II) to the PNAS office.
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Data deposition: The data reported in this paper have been deposited in the Gene Expression Omnibus (GEO) database, www.ncbi.nlm.nih.gov/geo (accessible through GEO series accession no. GSE4764).
- Abbreviations:
- PPs,
- Peyer’s patches;
- MLNs,
- mesenteric lymph nodes;
- PMNs,
- polymorphonuclear leukocytes;
- HDC,
- histidine decarboxylase;
- Hn,
- histamine receptor n;
- PI,
- postinfection;
- qRT-PCR,
- quantitative RT-PCR;
- PPI,
- proton pump inhibitor;
- cfu,
- colony-forming units;
- GO,
- gene ontology.
Abbreviations:
- © 2006 by The National Academy of Sciences of the USA
