Sterol-regulated transport of SREBPs from endoplasmic reticulum to Golgi: Oxysterols block transport by binding to Insig

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Fig. 5.
Fig. 5.

[3H]25-HC binding to Insig-2 mutants. Each assay tube, in a total volume of 100 μl of buffer A, contained 400 nM His10–Insig-2–FLAG (wild-type or the indicated mutant version), 25 mM phosphocholine chloride, and the indicated concentration of [3H]25-HC (152 dpm/fmol). After incubation for 4 h at room temperature, specifically bound [3H]25-HC was measured as described in Fig. 2C. Each data point is the average of duplicate assays.


This Article

  1. Published online before print April 11, 2007, doi: 10.1073/pnas.0700899104 PNAS April 17, 2007 vol. 104 no. 16 6511-6518
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