Isolation of Temperature-Sensitive Mutants of L-Cells*

  1. L. H. Thompson†,
  2. R. Mankovitz‡,
  3. R. M. Baker§,
  4. J. E. Till,
  5. L. Siminovitch, and
  6. G. F. Whitmore
  1. DEPARTMENT OF MEDICAL BIOPHYSICS, UNIVERSITY OF TORONTO
  2. DEPARTMENT OF MEDICAL CELL BIOLOGY, UNIVERSITY OF TORONTO
  3. THE ONTARIO CANCER INSTITUTE, TORONTO, ONTARIO

Abstract

Procedures are described for the isolation of conditional lethal mutants of mouse L-60T cells. The mutant lines were temperature sensitive by the following criteria: (a) colony-forming ability, (b) growth in suspension culture, and (c) rate of uptake of tritiated-thymidine.

Footnotes

  • ↵ † Requests for reprints may be sent to Dr. Thompson, The Ontario Cancer Institute, 500 Sherbourne Street, Toronto 284, Ontario, Canada.

  • ↵ ‡ Supported by a postdoctoral fellowship from the Medical Research Council of Canada.

  • ↵ § Supported by a postdoctoral fellowship from the U.S. Public Health Service.

  • ↵ * Supported by the Medical Research Council of Canada and the National Cancer Institute of Canada.

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  1. PNAS 1970-06 vol. 66 no. 2 377-384
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